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Biochemistry ResearchProgram Requirements

Course Offerings, Requirements, Choosing a Thesis Advisor, Completing Your Studies, Career Opportunites

Course Requirements. Ten credits are required for the major. All students are required to take two semesters of the 1 credit course, Topics in Microbiology (MMI 810 & Micro 811). These 2 credits may be used for either the major or the minor, with the approval of the thesis committee. At least three courses must come from the following:

Course

Title

Info

Micro 607

Advanced Microbial Genetics

3cr. Lectures cover mutagenesis, conjugation, transformation, transduction, plasmids, cloning and transposable elements. P:Micro/Genetics/Biochem 612 or cons inst.

Micro 612

Prokaryotic Molecular Biology

3cr. Molecular basis of bacterial physiology and genetics withemphasis on molecular mechanisms; topics include nucleic acid-protein interactions, transcription, translation, replication, recombination, regulation of Gene expression. P:Micro 370 or equiv &; Biochem 501 or equiv, or cons inst.

Micro 640

General Virology-Multiplication of Viruses

3cr. Bacterial and animal viruses, their structure, multiplication, and genetics. P:Intro courses in Micro, biochem & genetics.

Micro 668

Microbiology at Atomic Resolution

3 cr. Three-dimensional protein structures form the basis for discussions of high resolution microbiology; how particular problems are solved with given protein architectures and chemistries and how themes of protein structure are modified and recycled. P: Biochem (e.g. Biochem 501), molecular biol (e.g. Micro 526 or 612) required, one semester of physical chem preferred.

MMI 720

Topics in Immunology

3cr. Current questions in immunological research, designed to exploreselected topics in immunology, including genetic, cellular and molecular features of the immune system that are fundamental to the regulation of immune responses. P:MMI/AHABS/Micro 528 or equiv.

Micro 725

Microbial Physiology and Diversity

3cr. Diversity of morphology, physiology and ecology of bacteria and other microorganisms. Interrelationships between microorganisms and their environment. P:Micro 550 or equiv, and Biochem 501 or 601 or con reg & cons inst.

Micro 726

Regulation of Gene Expression in Prokaryotes

3cr. An intensive examination of a limited number of systems to illustrate the range of molecular mechanism utilized to control gene expression in bacteria. P:Micro/Genetics/Biochem 612 or cons inst.

MMI 740

Mechanisms of Microbial Pathogenesis

3cr. Lecture-discussion. Host-pathogen relationships in microbial diseases. Entry level course for infectious diseases sequence (see Med Micro 760, 790). P:Cons inst, MMI 301 or equiv & a course in immunology.

MMI 750

Host-Parasite Relationships

3cr Detailed study of the pathogenesis of vertebrate viral disease, stressing viral invasion, dissemination, mechanisms of disease production and resistance, and transmission. P:Oncol/Micro 640 or Vet Sci 513; and Micro, Vet Sci 528; or Cons Inst.

MMI 760

Molecular Genetics of Microbial Pathogenesis

3cr. The genetics and biochemistry of host cell adherence, cellular invasion and immune evasion by different human and animal microbial pathogens. The mechanisms and regulation of adhesins, toxins and antigenic variation. P:MMI 740 or equiv & cons inst.

MMI 773

Eukaryotic Microbial Pathogenesis

3cr. An advanced course focusing on the molecular, cellular, biochemical and pathogenicmechanisms found in fungal and protozoan pathogens of humans. A combination of lectures and student presentations will be employed. P. Cons. Inst. MMI 740 and Path 750 recommended.

MMI 790

Immunology of Infectious Disease

3 cr. Immunobiology and immunogenetics of resistance to infectious disease agents of man and animals; immunoregulatory mechanisms associated with evasion of host immunity. P: MM&I 720 or equiv, MM&I 740 or equiv, & cons inst.

The remaining credits may come from other Bacteriology or MMI courses approved by the Advising Committee or the Thesis Committee, excluding Micro. 731/MMI 901, MMI 900, and Micro. or MMI 699 and 990, or any other research, directed study, seminar or journal club course except as approved by the Steering Committee.

Course Offerings. Graduate courses are offered in microbial physiology, microbial and molecular genetics, microbial regulation and development, microbial diversity, industrial microbiology and biotechnology, immunology, microbial pathogenesis, virology, food microbiology, biochemistry and microbiology of soils, and the microbiology of plant pathogens. In addition, the departments periodically offer special topics courses. Examples of past special topics courses include: regulation of transcription, eucaryotic cell cycle, bacterial DNA replication, transcription termination and anti-termination, posttranscriptional regulation of gene expression, secondary metabolism and drug production, and practical aspects of HPLC, cell biology, and microscopy.

Requirements for the Minor. The Graduate School requires that each student fulfill a Ph.D. minor program. The minor can be completed by either taking 10 graduate credits from one discipline outside of the microbiology major, or by taking 10 credit hours distributed among courses from any department related to the individual training emphasis area. This can include Bacteriology or MMI courses not used for the major.

Grade Requirement. A student must maintain a B average in all courses to meet degree requirements. Grades of S areconsidered to be satisfactory; an incompletegrade must be removed during the following semester of residence or it will be converted to F. A student taking minor option A must satisfy the grade requirements of the minor department.

Department Seminar and/or Journal Club. Throughout their graduate study, students must register for and attend the weekly seminar, Micro. 731/MMI 901. Students are required to make at least one seminar presentation per academic year after achieving dissertator status. At least two of these presentations must be in Micro. 731/MMI 901.

Graduate Study Prior to Choosing a Thesis Adviser. During the week prior to the start of classes in the fall semester, each student must attend orientation activities and meet with the Advising Committee. The Committee reviews background course requirements, considers training emphasis area (if known) and devises a course of study for the first year. The Advising Committee also monitors rotations and is available for advice until a thesis advisor is identified. If a student is funded by certain training program grants, the student may also be assigned a specific adviser who represents that program until a thesis adviser is identified.

During the initial 6 months of graduate study, students complete at least 3 research rotations with 3 different program faculty or trainers. The length and timing of rotations will be decided by the individual student in consultation with the faculty with whom they are rotating, although 3-4 rotations of 1-2 months each is appropriate. Students may select a thesis adviser as early as Dec. 15, but no later than Feb. 21 of the first year. Deviation from the minimal number of rotations or dates for selection of a thesis adviser requires approval of the Advising Committee. A student who fails to select a thesis advisor by July 1 of the first year may be dismissed from the Program.

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Graduate Study after Choosing a Thesis Adviser. Formulation of the Thesis Committee. A committee consisting of five faculty members guides the student's progress towards the degree. At least one member must be outside the thesis adviser's department. Students form their committees at the end of their second semester and submit the names of the committee members to the Steering Committee for approval. At least 2 members of a student's thesis committee besides the thesis advisor must be members of the core faculty of the Bacteriology or Medical Microbiology and Immunology departments. Initial Meeting. The student may write a brief description of research planned for the next year (1-2) pages is sufficient) by June 15 of the first year, distribute the write-up to the committee and meet with the committee prior to August 15. At the meeting, the student's coursework for completion of degree requirements will be approved. Annual Meetings with Thesis Committee. Students are required to prepare a brief progress report and hold an annual meeting with the committee. The student is responsible for obtaining committee member signatures and turningthese in to the Program Office.

Completion of Graduate Studies. Teaching Requirement. As part of their training, students are required to perform two semesters of teaching. Waivers of one semester may be granted for special circumstances by the Steering Committee in consultation with the appropriate departmental administrator.

Preliminary Examination. Before the first day of spring semester classes in the third year in the Microbiology Ph.D. program, students will complete the requirements of the qualifying exam, which is centered around a research proposal. The exam consists of two parts, the written proposal and an oral defense by the student before the members of the Thesis Committee.

The Written Proposal (Part A). The student writes and submits the research proposal to the Thesis Committee. The subject matter of the proposal will coincide with the student's anticipated thesis research. The proposal will be prepared in the format of a National Institutes of Health research proposal, but approximately 15 double-spaced pages long. (A guideline for the written proposal will be provided.) The student should consult with others, including the thesis adviser and committee members, before and during the writing process. at least 6 weeks prior to the anticipated oral defense, the student will submit the proposal to the members of the committee. The committee will have two weeks to evaluate the proposal. During the third week, the student should meet with each member of the committee to learn his/her appraisal. Based on the these comments, the student will revise the proposal and resubmit it at least one week prior to the oral defense. If a committee member has reservations about the original or the revised proposal that are serious enough to make voting to "pass" unlikely, that member should notify the student and the thesis adviser of his/her concerns immediately. In such cases, the thesis adviser, after consultation with the other committee members may delay the defense to allow time for corrective actions.

The Oral Defense (Part B). The student will give a brief (20-30 min.) oral presentation describing the research proposal and then respond to questions raised by the members of the Thesis Committee. The questions will center around the research proposal, but may include any question relevant to it or to the expected proficiencies in microbiology enumerated by the Steering Committee. Following the examination, the Thesis Committee will decide whether the student (i) passes the exam unconditionally and proceeds to candidacy, (ii) passes the exam conditionally and is instructed to complete additional work to satisfy a perceived deficiency, or (iii) fails the exam.

Candidacy. After the student has passed the Preliminary Examination and all other degree requirements have been met, the Graduate School warrant certifying candidacy for the Ph.D. will be signed by the Thesis Committee and returned to the Graduate School.

Procedure for Appeal. A student who fails the Preliminary Examination may be offered a second opportunity to pass the qualifying examination or may be dismissed from the program. Appeals of a decision by the Thesis Committee must be made to the Steering Committee within two weeks or they will be final. The Steering Committee will make the final decision on an appeal. A student who has not satisfied the qualifying examination requirement within 36 months of entering the Ph.D. program will be dropped from the program, except by appeal in writing to the Steering Committee, which will make the final decision.

Thesis and Final Defense. Prior to graduation, every student must submit a thesis based on original and significant observations. Six months prior to the expected defense date, the student will meet with the Thesis Committee and obtain approval of the thesis prospectus. Students are required to present a seminar on his/her dissertation research, which is followed directly by the final examination. The final examination will be an oral defense of the thesis to the Thesis Committee. One month prior to the scheduled oral defense, the student must obtain the Ph.D. warrant from the Graduate School. For the students to pass the final exam, four of the five committee members must sign to affirm passage.

Career Opportunities for Program Graduates. UW-Madison Microbiology graduates compete successfully for positions in the nation's most prestigious academic institutions, industrial and clinical laboratories, and government agencies. The Program maintains a comprehensive Career Resource Guide and Job Bulletin with updated information on career and employment opportunities. If you need assistance with career and job exploration, as well as help with developing your CV, please contact Katy France in 1328 Microbial Sciences or at 608-262-2975.

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